Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Stat3

Cell type

Cell type Class
Neural
Cell type
Spinal Cord
MeSH Description
A cylindrical column of tissue that lies within the vertebral canal. It is composed of WHITE MATTER and GRAY MATTER.

Attributes by original data submitter

Sample

source_name
Spinal cord dissociated cells
cell type
Spinal cord dissociated cells
strain
C57BL/6
chip antibody
Rabbit anti-STAT3_(sc-482x)
treatment
Thoracic (T9) moderate contusion injury for 7 days

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (IP) was performed using STAT3 antibody (sc-482x, Santa Cruz Biotechnology). IP DNA and input DNA were extracted using phenol:chloroform as described in our previous study Libraries were constructed with the DNA SMART ChIP-Seq Kit (634865, Clontech) by following the manufacturer's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
3331228
Reads aligned (%)
48.8
Duplicates removed (%)
30.7
Number of peaks
51 (qval < 1E-05)

mm9

Number of total reads
3331228
Reads aligned (%)
48.7
Duplicates removed (%)
30.7
Number of peaks
46 (qval < 1E-05)

Base call quality data from DBCLS SRA