Antigen

Antigen Class

TFs and others

Antigen

Stat3

Cell type

Cell type Class

Neural

Cell type

Spinal Cord

MeSH Description

A cylindrical column of tissue that lies within the vertebral canal. It is composed of WHITE MATTER and GRAY MATTER.

Sample

source_name

Spinal cord dissociated cells

cell type

Spinal cord dissociated cells

strain

C57BL/6

chip antibody

Rabbit anti-STAT3_(sc-482x)

treatment

Thoracic (T9) moderate contusion injury for 7 days

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation (IP) was performed using STAT3 antibody (sc-482x, Santa Cruz Biotechnology). IP DNA and input DNA were extracted using phenol:chloroform as described in our previous study Libraries were constructed with the DNA SMART ChIP-Seq Kit (634865, Clontech) by following the manufacturer's instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

3331228

Reads aligned (%)

48.8

Duplicates removed (%)

30.7

Number of peaks

51 (qval < 1E-05)

mm9

Number of total reads

3331228

Reads aligned (%)

48.7

Duplicates removed (%)

30.7

Number of peaks

46 (qval < 1E-05)